Characterization of Phenotypic Switching in Cryptococcus neoformans Biofilms
نویسندگان
چکیده
منابع مشابه
Genetic and phenotypic characterization of capsule mutants of Cryptococcus neoformans.
Stable mutants with reduced capacity to produce capsules were isolated from suspensions of Cryptococcus neoformans after treatment of the wild type with a mutagen. The mutants could be assigned one of two phenotypes, hypocapsular or acapsular. Hypocapsular mutants were immunochemically and physicochemically indistinguishable from the wild type, whereas acapsular mutants lacked a major capsular ...
متن کاملPhenotypic switching of Cryptococcus neoformans occurs in vivo and influences the outcome of infection.
Phenotypic switching has been linked to the virulence of many pathogens, including fungi. However, it has not been conclusively shown to occur in vivo or to influence the outcome of infection. Cryptococcus neoformans undergoes phenotypic switching in vitro to colony types that differ in their virulence in mice. In this study, we asked whether C. neoformans undergoes phenotypic switching in vivo...
متن کاملSusceptibility of Cryptococcus neoformans biofilms to antifungal agents in vitro.
Microbial biofilms contribute to virulence and resistance to antibiotics by shielding microbial cells from host defenses and antimicrobial drugs, respectively. Cryptococcus neoformans was demonstrated to form biofilms in polystyrene microtiter plates. The numbers of CFU of disaggregated biofilms, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide reducti...
متن کاملIt infects me, it infects me not: phenotypic switching in the fungal pathogen Cryptococcus neoformans.
Phenotypic switching has been observed in both prokaryotes and eukaryotes and involves stochastic switching between two or more alternative and heritable phenotypes, usually distinguished by surface antigenicity. This strategy, generally adopted by pathogens to escape recognition by the immune system and to adapt to a hostile host environment, results from spontaneous alterations in gene expres...
متن کاملCryptococcus neoformans variants generated by phenotypic switching differ in virulence through effects on macrophage activation.
Macrophages have a central role in the pathogenesis of cryptococcosis since they are an important line of defense, serve as a site for fungal replication, and also can contribute to tissue damage. The objective of this study was to investigate the interaction of macrophages with cells from smooth-colony variants (SM) and mucoid-colony variants (MC) arising from phenotypic switching of Cryptococ...
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ژورنال
عنوان ژورنال: Mycopathologia
سال: 2008
ISSN: 0301-486X,1573-0832
DOI: 10.1007/s11046-008-9133-0